human pancreatic cancer cell lines aspc 1  (ATCC)

Journal: Cell Death & Disease

Article Title: Adipogenic transdifferentiation reprograms EMT-high PDAC cells into a post-mitotic adipocyte-like state and limits metastasis

doi: 10.1038/s41419-026-08613-4

Figure Lengend Snippet: A Graphic scheme of adipogenic transdifferentiation induction protocol and microphotographs of control and transdifferentiated AsPC-1 cells at day 0, 3, 7, and 10; Rectangle dialog boxes magnify the field of view from the two groups. B Visualization of accumulated lipid droplets by oil red O staining in control and transdifferentiated AsPC-1 cells on day 10 after induction. Five random fields of view were used for quantification, values were presented as the mean ± SD ( n = 5 per group) and compared by Student’s t -test. C Relative mRNA expression levels (normalized to ACTB expression from the same individual sample) of adipocyte markers adiponectin, CEBPA, PPARG , and FABP4 in eight human pancreatic cell lines. Values were presented as the mean ± SD ( n = 3 per group) and compared by Student’s t -test. Abbreviation: SD Standard deviation.

Article Snippet: The human pancreatic cancer cell lines AsPC-1 (RRID: CVCL_0152), CFPAC-1 (RRID: CVCL_1119), Mia PaCa-2 (Cat#SCSP-568), PANC-1 (Cat#SCSP-535), SW-1990 (Cat#SCSP-5279) were obtained from the National Collection of Authenticated Cell Cultures, Capan-1 (Cat#HTB-79) were obtained from the American Type Culture Collection, PaTu 8988t was obtained from Procell (Cat#CL-0579).

Techniques: Control, Staining, Expressing, Standard Deviation

Journal: Cell Death & Disease

Article Title: Adipogenic transdifferentiation reprograms EMT-high PDAC cells into a post-mitotic adipocyte-like state and limits metastasis

doi: 10.1038/s41419-026-08613-4

Figure Lengend Snippet: Visualization and quantification of immunostaining of CEBPA ( A ), PPARG ( B ), and FABP4 ( C ) in control and transdifferentiated AsPC-1 cells on day 10. The protein expression levels were quantified by integrated intensity and positive area of five random fields of view. Values were presented as the mean ± SD ( n = 5 per group) and compared by Student’s t -test. D The protein expression levels of CEBPA and PPARG as adipogenesis markers and GAPDH as reference marker in AsPC-1. Abbreviation: SD standard deviation.

Article Snippet: The human pancreatic cancer cell lines AsPC-1 (RRID: CVCL_0152), CFPAC-1 (RRID: CVCL_1119), Mia PaCa-2 (Cat#SCSP-568), PANC-1 (Cat#SCSP-535), SW-1990 (Cat#SCSP-5279) were obtained from the National Collection of Authenticated Cell Cultures, Capan-1 (Cat#HTB-79) were obtained from the American Type Culture Collection, PaTu 8988t was obtained from Procell (Cat#CL-0579).

Techniques: Immunostaining, Control, Expressing, Marker, Standard Deviation

Journal: Cell Death & Disease

Article Title: Adipogenic transdifferentiation reprograms EMT-high PDAC cells into a post-mitotic adipocyte-like state and limits metastasis

doi: 10.1038/s41419-026-08613-4

Figure Lengend Snippet: A Adiponectin secretion levels in control and transdifferentiated AsPC-1 cells after 10 days’ induction. Values were presented as the mean ± SD ( n = 6 per group) and compared by Student’s t -test. B Glycerol release from control and transdifferentiated AsPC-1 cells after 10 days’ induction. Isoproterenol was used to stimulate cellular lipolysis. Values were presented as the mean ± SD ( n = 5 per group) and compared by Student’s t -test. C Cell proliferation of control and transdifferentiated AsPC-1 was monitored for 6 days during adipogenesis induction. Values were presented as the mean ± SD ( n = 5 per group) and compared by Student’s t -test. D – F Cell cycle analysis of control D and transdifferentiated E AsPC-1 after 10 days’ induction. Percentages of cell numbers of G1, S, and G2 phases were plotted as the mean ± SD ( n = 3 per group) and compared by student’s t -test. G – I Representative fields of Transwell migration and invasion assays for control and transdifferentiated AsPC-1 cells after 10 days’ induction. The Y axis represented relative migration or invasion cell percentage, normalized to the average of control group (set as 100%). Quantification was performed by analyzing five random fields ( H , I ), and values were presented as the mean ± SD ( n = 5 per group) and compared by Student’s t -test. The numerous tiny dots are the pores of the Transwell membrane. J Relative mRNA levels of EMT markers ( E-cadherin and vimentin ) and EMT-TFs ( Snail1, Snail2, Twist1, Twist2, ZEB1 , and ZEB2 ) in control and transdifferentiated AsPC-1 cells. Values were presented as the mean ± SD ( n = 3 per group) and compared by Student’s t -test. Abbreviation: SD standard deviation, EMT epithelial-mesenchymal transition, TF Transcription factor.

Article Snippet: The human pancreatic cancer cell lines AsPC-1 (RRID: CVCL_0152), CFPAC-1 (RRID: CVCL_1119), Mia PaCa-2 (Cat#SCSP-568), PANC-1 (Cat#SCSP-535), SW-1990 (Cat#SCSP-5279) were obtained from the National Collection of Authenticated Cell Cultures, Capan-1 (Cat#HTB-79) were obtained from the American Type Culture Collection, PaTu 8988t was obtained from Procell (Cat#CL-0579).

Techniques: Control, Cell Cycle Assay, Migration, Membrane, Standard Deviation